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Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:one hundred dilution. Word that the facet chain has three methylene groups, so that though the terminal amino group shall be charged below physiological conditions, the aspect chain does have important hydrophobic character. Incubate the membrane for 20 min and then wash with DMF to take away uncoupled amino acids.
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