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Be aware that the aspect chain has three methylene teams, in order that though the terminal amino group will likely be charged underneath physiological conditions, the side chain does have significant hydrophobic character. Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution. Incubate the membrane for 20 min and then wash with DMF to take away uncoupled amino acids.
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